control scrambled shrna sh nc Search Results


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Shanghai GenePharma matched shrna control sh-nc
Matched Shrna Control Sh Nc, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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VectorBuilder GmbH oe-dnmt3b
Primer used for RT-qPCR
Oe Dnmt3b, supplied by VectorBuilder GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Shanghai GenePharma lentiviruses designed for overexpressing mettl16 and senp3
Primer used for RT-qPCR
Lentiviruses Designed For Overexpressing Mettl16 And Senp3, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ribobio co shrna control sh-nc
Primer used for RT-qPCR
Shrna Control Sh Nc, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Broad Institute Inc shrna control (sh-scramble)
Primer used for RT-qPCR
Shrna Control (Sh Scramble), supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Shanghai GenePharma adenovirus expressing shrna (sh)-negative control (nc)
Primer used for RT-qPCR
Adenovirus Expressing Shrna (Sh) Negative Control (Nc), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Shanghai GenePharma control nonspecific shrnas (sh-nc)
Primer used for RT-qPCR
Control Nonspecific Shrnas (Sh Nc), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Shanghai GenePharma nontargeted scrambled control shrna–nc
Primer used for RT-qPCR
Nontargeted Scrambled Control Shrna–Nc, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SunBio Inc lentivirus containing negative control shrna (sh-nc
Effects of FGD5-AS1 inhibition on gastric cancer cell proliferation and 5-FU chemoresistance. (A) Two gastric cancer cell lines, SGC-7901 and MKN-28 were transduced with a <t>lentivirus</t> containing human lncRNA FGD5-AS1 sh_RNA (sh_FGD5AS1), or a lentivirus containing negative control shRNA (sh-NC). After transduction was stabilized, qRT-PCR was applied to compare FGD5-AS1 expression levels (* P < 0.05). (B) A 5-day MTT assay was used to assess in vitro proliferation rates between SGC-7901 and MKN-28 cells transduced with sh-NC and those transduced with sh-FGD5AS1 (** P < 0.05). (C) Lentiviral-transduced SGC-7901 and MKN-28 cells were treated with 0, 5, 10, 20, 50, or 100 μg/mL 5-FU for 24 h, followed by an MTT assay to compare 5-FU chemoresistance between those transduced with sh-NC and those transduced with sh-FGD5AS1 (** P < 0.05). (D) Lentiviral-transduced SGC-7901 cells were subcutaneously inoculated (1 × 10 6 /injection) into the flanks of 2-month-old female athymic mice for 5 weeks. The in vivo tumor volumes (mm 3 ) were compared between those transduced with sh-NC and those transduced with sh-FGD5AS1 (** P < 0.05). (E) After the in vivo tumorigenicity assay, SGC-7901 tumors were retrieved and compared directly.
Lentivirus Containing Negative Control Shrna (Sh Nc, supplied by SunBio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ribobio co nonspecific shrnas
Effects of FGD5-AS1 inhibition on gastric cancer cell proliferation and 5-FU chemoresistance. (A) Two gastric cancer cell lines, SGC-7901 and MKN-28 were transduced with a <t>lentivirus</t> containing human lncRNA FGD5-AS1 sh_RNA (sh_FGD5AS1), or a lentivirus containing negative control shRNA (sh-NC). After transduction was stabilized, qRT-PCR was applied to compare FGD5-AS1 expression levels (* P < 0.05). (B) A 5-day MTT assay was used to assess in vitro proliferation rates between SGC-7901 and MKN-28 cells transduced with sh-NC and those transduced with sh-FGD5AS1 (** P < 0.05). (C) Lentiviral-transduced SGC-7901 and MKN-28 cells were treated with 0, 5, 10, 20, 50, or 100 μg/mL 5-FU for 24 h, followed by an MTT assay to compare 5-FU chemoresistance between those transduced with sh-NC and those transduced with sh-FGD5AS1 (** P < 0.05). (D) Lentiviral-transduced SGC-7901 cells were subcutaneously inoculated (1 × 10 6 /injection) into the flanks of 2-month-old female athymic mice for 5 weeks. The in vivo tumor volumes (mm 3 ) were compared between those transduced with sh-NC and those transduced with sh-FGD5AS1 (** P < 0.05). (E) After the in vivo tumorigenicity assay, SGC-7901 tumors were retrieved and compared directly.
Nonspecific Shrnas, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Shanghai GenePharma sirna-cd164 with 2'-o-me modifications
Effects of FGD5-AS1 inhibition on gastric cancer cell proliferation and 5-FU chemoresistance. (A) Two gastric cancer cell lines, SGC-7901 and MKN-28 were transduced with a <t>lentivirus</t> containing human lncRNA FGD5-AS1 sh_RNA (sh_FGD5AS1), or a lentivirus containing negative control shRNA (sh-NC). After transduction was stabilized, qRT-PCR was applied to compare FGD5-AS1 expression levels (* P < 0.05). (B) A 5-day MTT assay was used to assess in vitro proliferation rates between SGC-7901 and MKN-28 cells transduced with sh-NC and those transduced with sh-FGD5AS1 (** P < 0.05). (C) Lentiviral-transduced SGC-7901 and MKN-28 cells were treated with 0, 5, 10, 20, 50, or 100 μg/mL 5-FU for 24 h, followed by an MTT assay to compare 5-FU chemoresistance between those transduced with sh-NC and those transduced with sh-FGD5AS1 (** P < 0.05). (D) Lentiviral-transduced SGC-7901 cells were subcutaneously inoculated (1 × 10 6 /injection) into the flanks of 2-month-old female athymic mice for 5 weeks. The in vivo tumor volumes (mm 3 ) were compared between those transduced with sh-NC and those transduced with sh-FGD5AS1 (** P < 0.05). (E) After the in vivo tumorigenicity assay, SGC-7901 tumors were retrieved and compared directly.
Sirna Cd164 With 2' O Me Modifications, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+scrambled+shrna+sh+nc/pmc12277295-69-4-0?v=Shanghai+GenePharma
Average 90 stars, based on 1 article reviews
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Shanghai GenePharma pkcε short hairpin (sh)rnas scramble control shrna
Effects of FGD5-AS1 inhibition on gastric cancer cell proliferation and 5-FU chemoresistance. (A) Two gastric cancer cell lines, SGC-7901 and MKN-28 were transduced with a <t>lentivirus</t> containing human lncRNA FGD5-AS1 sh_RNA (sh_FGD5AS1), or a lentivirus containing negative control shRNA (sh-NC). After transduction was stabilized, qRT-PCR was applied to compare FGD5-AS1 expression levels (* P < 0.05). (B) A 5-day MTT assay was used to assess in vitro proliferation rates between SGC-7901 and MKN-28 cells transduced with sh-NC and those transduced with sh-FGD5AS1 (** P < 0.05). (C) Lentiviral-transduced SGC-7901 and MKN-28 cells were treated with 0, 5, 10, 20, 50, or 100 μg/mL 5-FU for 24 h, followed by an MTT assay to compare 5-FU chemoresistance between those transduced with sh-NC and those transduced with sh-FGD5AS1 (** P < 0.05). (D) Lentiviral-transduced SGC-7901 cells were subcutaneously inoculated (1 × 10 6 /injection) into the flanks of 2-month-old female athymic mice for 5 weeks. The in vivo tumor volumes (mm 3 ) were compared between those transduced with sh-NC and those transduced with sh-FGD5AS1 (** P < 0.05). (E) After the in vivo tumorigenicity assay, SGC-7901 tumors were retrieved and compared directly.
Pkcε Short Hairpin (Sh)rnas Scramble Control Shrna, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Primer used for RT-qPCR

Journal: BMC Cancer

Article Title: Interaction between DNMT3B and MYH11 via hypermethylation regulates gastric cancer progression

doi: 10.1186/s12885-021-08653-3

Figure Lengend Snippet: Primer used for RT-qPCR

Article Snippet: The overexpressed (oe) DNA plasmids oe-MYH11, oe-TNFRSF14, oe-DNMT3B and control oe-negative control (NC) used for cell transfection were from VectorBuilder (Guangzhou, Guangdong, China).

Techniques:

DNMT3B upregulation is responsible for the hypermethylation of the MYH11 promoter. A , the expression of DNMT3A and DNMT3B in GC was queried in StarBase Pan-cancer platform; B , the enrichment ability of DNMT3A and DNMT3B on MYH11 promoter examined by ChIP-qPCR; C , DNMT3B mRNA expression in GC tumor tissues and their adjacent tissues by RT-qPCR; D , correlation of DNMT3B expression with MYH11 promoter methylation levels in tumor tissues analyzed by Pearson’s correlation analysis (r = 0.623, p < 0.001); E , correlation of DNMT3B expression with MYH11 expression in tumor tissues (r = − 0.609, p < 0.001); F , transfection efficiency of oe-DNMT3B in GC cells by RT-qPCR; G , the effects of DNMT3B overexpression on the methylation level of MYH11 promoter examined by qMSP; H , effects of DNMT3B overexpression on MYH11 expression by RT-qPCR. Each assay was performed at least three times. Statistical significance was analyzed by paired t test (panel C) or two-way ANOVA (panels B, F, G and H) and Tukey’s multiple range tests

Journal: BMC Cancer

Article Title: Interaction between DNMT3B and MYH11 via hypermethylation regulates gastric cancer progression

doi: 10.1186/s12885-021-08653-3

Figure Lengend Snippet: DNMT3B upregulation is responsible for the hypermethylation of the MYH11 promoter. A , the expression of DNMT3A and DNMT3B in GC was queried in StarBase Pan-cancer platform; B , the enrichment ability of DNMT3A and DNMT3B on MYH11 promoter examined by ChIP-qPCR; C , DNMT3B mRNA expression in GC tumor tissues and their adjacent tissues by RT-qPCR; D , correlation of DNMT3B expression with MYH11 promoter methylation levels in tumor tissues analyzed by Pearson’s correlation analysis (r = 0.623, p < 0.001); E , correlation of DNMT3B expression with MYH11 expression in tumor tissues (r = − 0.609, p < 0.001); F , transfection efficiency of oe-DNMT3B in GC cells by RT-qPCR; G , the effects of DNMT3B overexpression on the methylation level of MYH11 promoter examined by qMSP; H , effects of DNMT3B overexpression on MYH11 expression by RT-qPCR. Each assay was performed at least three times. Statistical significance was analyzed by paired t test (panel C) or two-way ANOVA (panels B, F, G and H) and Tukey’s multiple range tests

Article Snippet: The overexpressed (oe) DNA plasmids oe-MYH11, oe-TNFRSF14, oe-DNMT3B and control oe-negative control (NC) used for cell transfection were from VectorBuilder (Guangzhou, Guangdong, China).

Techniques: Expressing, Quantitative RT-PCR, Methylation, Transfection, Over Expression

Mechanism diagram. Overexpression of DNMT3B in GC inhibited MYH11 expression by promoting methylation of the MYH11 promoter, thereby attenuating the repressive effect of MYH11 on TNFRSF14 transcriptional activity and promoting GC progression

Journal: BMC Cancer

Article Title: Interaction between DNMT3B and MYH11 via hypermethylation regulates gastric cancer progression

doi: 10.1186/s12885-021-08653-3

Figure Lengend Snippet: Mechanism diagram. Overexpression of DNMT3B in GC inhibited MYH11 expression by promoting methylation of the MYH11 promoter, thereby attenuating the repressive effect of MYH11 on TNFRSF14 transcriptional activity and promoting GC progression

Article Snippet: The overexpressed (oe) DNA plasmids oe-MYH11, oe-TNFRSF14, oe-DNMT3B and control oe-negative control (NC) used for cell transfection were from VectorBuilder (Guangzhou, Guangdong, China).

Techniques: Over Expression, Expressing, Methylation, Activity Assay

Effects of FGD5-AS1 inhibition on gastric cancer cell proliferation and 5-FU chemoresistance. (A) Two gastric cancer cell lines, SGC-7901 and MKN-28 were transduced with a lentivirus containing human lncRNA FGD5-AS1 sh_RNA (sh_FGD5AS1), or a lentivirus containing negative control shRNA (sh-NC). After transduction was stabilized, qRT-PCR was applied to compare FGD5-AS1 expression levels (* P < 0.05). (B) A 5-day MTT assay was used to assess in vitro proliferation rates between SGC-7901 and MKN-28 cells transduced with sh-NC and those transduced with sh-FGD5AS1 (** P < 0.05). (C) Lentiviral-transduced SGC-7901 and MKN-28 cells were treated with 0, 5, 10, 20, 50, or 100 μg/mL 5-FU for 24 h, followed by an MTT assay to compare 5-FU chemoresistance between those transduced with sh-NC and those transduced with sh-FGD5AS1 (** P < 0.05). (D) Lentiviral-transduced SGC-7901 cells were subcutaneously inoculated (1 × 10 6 /injection) into the flanks of 2-month-old female athymic mice for 5 weeks. The in vivo tumor volumes (mm 3 ) were compared between those transduced with sh-NC and those transduced with sh-FGD5AS1 (** P < 0.05). (E) After the in vivo tumorigenicity assay, SGC-7901 tumors were retrieved and compared directly.

Journal: Frontiers in Genetics

Article Title: Long Non-coding RNA FGD5-AS1 Regulates Cancer Cell Proliferation and Chemoresistance in Gastric Cancer Through miR-153-3p/CITED2 Axis

doi: 10.3389/fgene.2020.00715

Figure Lengend Snippet: Effects of FGD5-AS1 inhibition on gastric cancer cell proliferation and 5-FU chemoresistance. (A) Two gastric cancer cell lines, SGC-7901 and MKN-28 were transduced with a lentivirus containing human lncRNA FGD5-AS1 sh_RNA (sh_FGD5AS1), or a lentivirus containing negative control shRNA (sh-NC). After transduction was stabilized, qRT-PCR was applied to compare FGD5-AS1 expression levels (* P < 0.05). (B) A 5-day MTT assay was used to assess in vitro proliferation rates between SGC-7901 and MKN-28 cells transduced with sh-NC and those transduced with sh-FGD5AS1 (** P < 0.05). (C) Lentiviral-transduced SGC-7901 and MKN-28 cells were treated with 0, 5, 10, 20, 50, or 100 μg/mL 5-FU for 24 h, followed by an MTT assay to compare 5-FU chemoresistance between those transduced with sh-NC and those transduced with sh-FGD5AS1 (** P < 0.05). (D) Lentiviral-transduced SGC-7901 cells were subcutaneously inoculated (1 × 10 6 /injection) into the flanks of 2-month-old female athymic mice for 5 weeks. The in vivo tumor volumes (mm 3 ) were compared between those transduced with sh-NC and those transduced with sh-FGD5AS1 (** P < 0.05). (E) After the in vivo tumorigenicity assay, SGC-7901 tumors were retrieved and compared directly.

Article Snippet: A lentivirus containing human lncRNA FGD5-AS1 short hairpin RNA (shRNA) (sh_FGD5AS1), and a lentivirus containing negative control shRNA (sh-NC) were purchased from SunBio (SunBio Medical Biotechnology, Shanghai, China).

Techniques: Inhibition, Transduction, Negative Control, shRNA, Quantitative RT-PCR, Expressing, MTT Assay, In Vitro, Injection, In Vivo, Tumorigenicity Assay